Lys-C, Mass Spec Grade from Promega

Product Specs
ItemLys-C, Mass Spec Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberVA1170
Quantity20microg
TypeEnzyme

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Multi-modal proteomic characterization of lysosomal function and proteostasis in progranulin-deficient neuronsMolecular NeurodegenerationNovember 16, 2023Saadia Hasan, Michael S. Fernandopulle, Stewart W. Humble, Ashley M. Frankenfield, Haorong Li, Ryan Prestil, Kory R. Johnson, Brent J. Ryan, Richard Wade-Martins, Michael E. Ward, et al.(IAA) for 30 min in a ThermoMixer shaking at 800 g at 37 °C. Proteins were digested with LysC (Promega #VA1170) at 1:30 (enzyme: protein) ratio for 16 h at 37 °C and quenched with 10% trifluoroacetic acid (TFA)

(2) Analysis of the N-glycosylation profiles of the spike proteins from the Alpha, Beta, Gamma, and Delta variants of SARS-CoV-2Analytical and Bioanalytical ChemistryAugust 1, 2023Dongxia Wang, Jakub Baudys, Sarah H. Osman, John R. Barrtrypsin (Cat#XV528X, Mass Spectrometry Grade), chymotrypsin (Cat#V1061, Sequencing Grade), Lys-C (Cat#VA1170, Mass Spectrometry Grade), and Asp-N (Cat#V162A, Sequencing Grade), were obtained from Promega (Madison,

(3) Flt1 produced by lung endothelial cells impairs ATII cell transdifferentiation and repair in pulmonary fibrosisCell Death & DiseaseJuly 15, 2023Maria Concetta Volpe, Giulio Ciucci, Giulia Zandomenego, Roman Vuerich, Nadja Anneliese Ruth Ring, Simone Vodret, Francesco Salton, Pietro Marchesan, Luca Braga, Thomas Marcuzzo, et al.as described [18]. Mass spectrometry Protein samples (20 μg) were digested with Lys-C (Promega, #VA1170), desalted and lyophilized for LC-MS/MS analysis on an Easy-nLC 1200 system (Thermo Fisher Scientific). Statistical

(4) Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cellsSTAR ProtocolsMarch 1, 2023Beatriz Calle, Edgar Gonzalez-Rodriguez, Keira E. Mahoney, Anna Cioce, Ganka Bineva-Todd, Omur Y. Tastan, Chloe Roustan, Helen Flynn, Stacy A. Malaker, Benjamin Schumann,Thermo Fisher Scientific,Thermo Fisher Scientific Cat# 15338100 "Lys-C, Mass Spec Grade","Promega (Madison, USA)",Promega Cat# VA1170 Ni-NTA® Agarose beads,"Qiagen (Hilden, Germany)",Qiagen Cat# 30210 Nuclease free water,Thermo Fisher

(5) Multi-modal Proteomic Characterization of Lysosomal Function and Proteostasis in Progranulin-Deficient NeuronsbioRxiv : the preprint server for biologyFebruary 24, 2023Hasan S, Fernandopulle MS, Humble SW, Frankenfield AM, Li H, Prestil R, Johnson KR, Ryan BJ, Wade-Martins R, Ward ME, et al.(IAA) for 30 min in a ThermoMixer shaking at 800 g at 37°C. Proteins were digested with LysC (Promega #VA1170) at 1:30 (enzyme:protein) ratio for 16 hours at 37°C and quenched with 10% trifluoroacetic acid (TFA)

(6) Protein Expression of Amino Acid Transporters Is Altered in Isolated Cerebral Microvessels of 5xFAD Mouse Model of Alzheimer’s DiseaseMolecular NeurobiologyFebruary 1, 2023Elena Puris, Liudmila Saveleva, Izaque de Sousa Maciel, Katja M. Kanninen, Seppo Auriola, Gert FrickerTosylphenylalanylchloromethyl ketone-treated trypsin (VA9000), Protease-Max surfactant (V2072), and lysyl endopeptidase (Lys-C, VA1170) were purchased from Promega (Madison, WI, USA). A mixture of standards (product number MSK-A2-1.2)

(7) Increasing the throughput of sensitive proteomics by plexDIANature BiotechnologyJanuary 1, 2023Jason Derks, Andrew Leduc, Georg Wallmann, R. Gray Huffman, Matthew Willetts, Saad Khan, Harrison Specht, Markus Ralser, Vadim Demichev, Nikolai Slavov0.3 U μl−1 of Trypsin Gold (Promega, V5280) to 1:25 ratio of substrate:protease and LysC (Promega, VA1170) to 1:40 ratio of substrate:protease and then incubated at 37 °C for 18 hours. E. coli and S. cerevisiae

(8) Co-display of diverse spike proteins on nanoparticles broadens sarbecovirus neutralizing antibody responsesiScienceDecember 22, 2022Mitch Brinkkemper, Tim S. Veth, Philip J.M. Brouwer, Hannah Turner, Meliawati Poniman, Judith A. Burger, Joey H. Bouhuijs, Wouter Olijhoek, Ilja Bontjer, Jonne L. Snitselaar, et al.L4509 Tris(2-carboxyethyl)phosphine,Sigma-Aldrich,Cat# SBR00051 Chloroacetamide,Sigma-Aldrich,Cat# C0267 Trypsin,Promega,Cat# V5117 LysC,Promega,Cat# VA1170 SARS-CoV peptide 1 DGIYFAATEK,Pepscan,N/A SARS-CoV peptide 2 AISQIQESLTTTSTALGK,Pepscan,N/A SARS-CoV-2

(9) Increased Expression and Activity of Brain Cortical cPLA2 Due to Chronic Lipopolysaccharide Administration in Mouse Model of Familial Alzheimer’s DiseasePharmaceuticsNovember 10, 2022Mikko Gynther, Mariana Leal Estrada, Sanna Loppi, Paula Korhonen, Katja M. Kanninen, Tarja Malm, Jari Koistinaho, Seppo Auriola, Gert Fricker, Elena Purisketone-treated trypsin (#VA9000), Protease Max surfactant (#V2072), and lysyl endopeptidase (Lys-C, #VA1170) were purchased from Promega (Madison, WI, USA). BioRad DC Protein Assay was purchased from EnVision

(10) Targeting pancreatic cancer by TAK-981: a SUMOylation inhibitor that activates the immune system and blocks cancer cell cycle progression in a preclinical modelGutNovember 1, 2022Sumit Kumar, Mark J A Schoonderwoerd, Jessie S Kroonen, Ilona J de Graaf, Marjolein Sluijter, Dina Ruano, RomÃ¡n GonzÃ¡lez-Prieto, Matty Verlaan-de Vries, Jasper Rip, Ramon Arens, et al.samples were alkylated using 5 mM chloroacetamide. Proteins were first digested with Lys-C (Promega, VA1170) in a 1:100 enzyme-to-protein ratio for 5 hours. Peptides were diluted with 50 mM ammonium bicarbonate

(11) Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cellsiScienceMay 20, 2022Iwasaki M, Kawahara Y, Okubo C, Yamakawa T, Nakamura M, Tabata T, Nishi Y, Narita M, Ohta A, Saito H, et al.sarcosinate (SLS),WAKO,Cat# 192-10382 Sucrose,WAKO,Cat# 195-07925 "Lys-C, Mass Spec Grade",Promega,Cat# VA1170 Sequencing Grade Modified Trypsin (Lyophilized),Promega,Cat# V5111 Ethyl acetate,WAKO,Cat# 051-00356 Acetonitrile,WAKO,Cat#

(12) Mitochondrial calcium uniporter deletion prevents painful diabetic neuropathy by restoring mitochondrial morphology and dynamicsPainMarch 1, 2022Dale S. George et al.iodoacetamide. Proteins were then digested for 3 hours with 2 μg of endoproteinase LysC (Promega Cat# VA1170) and subsequently digested overnight with 2 μg of trypsin (Promega V5280). The digest was then acidified

(13) 3-hydroxy-L-kynurenamine is an immunomodulatory biogenic amineNature communicationsJuly 21, 2021Clement CC, D'Alessandro A, Thangaswamy S, Chalmers S, Furtado R, Spada S, Mondanelli G, Ianni F, Gehrke S, Gargaro M, et al.Proteinase inhibitor cocktail (cat# 04693116001) was purchased from Roche. Trypsin (cat# V5111), Lys-C (cat# VA1170), and GluC (cat# V1651), all sequencing grade purity were purchased from Promega (Madison, WI, USA). Synthesis

(14) PHAROH lncRNA regulates Myc translation in hepatocellular carcinoma via sequestering TIAReLifeMay 18, 2021Allen T Yu, Carmen Berasain, Sonam Bhatia, Keith Rivera, Bodu Liu, Frank Rigo, Darryl J Pappin, David L Spectorand 10 mM iodoacetamide for 30 min at 55°C, then digested overnight at 37°C with 1 μg Lys-C (Promega, VA1170) and dried in vacuo. Peptides were then reconstituted in 50 μl of 0.5 M TEAB/70% ethanol and labeled

(15) Pleiotropic consequences of metabolic stress for the major histocompatibility complex class II molecule antigen processing and presentation machineryImmunityApril 13, 2021Clement CC, Nanaware PP, Yamazaki T, Negroni MP, Ramesh K, Morozova K, Thangaswamy S, Graves A, Kim HJ, Li TW, et al.11088858001 XCR1-Brilliant Violet 510,Biolegend,Catalog # 148218 Endoproteinase Lys-C,Promega,Cat.# VA1170 Trypsin,Promega,Catalog # V5111 Glu-C,Promega,Catalog # V1651 Acetonitrile Optima™ LC/MS,Fisher Scientific,Catalog

(16) PADI2‐Catalyzed MEK1 Citrullination Activates ERK1/2 and Promotes IGF2BP1‐Mediated SOX2 mRNA Stability in Endometrial CancerAdvanced ScienceMarch 1, 2021Teng Xue et al.re‐acting with 15 mm iodoacetamide for 45 min in the dark. The sample was then digested with Lys‐C (VA1170, Promega) at 37 °C for 16 h. Peptide samples were then desalted and lyophilized. The sample were then

(17) One-shot analysis of translated mammalian lncRNAs with AHARIBOeLifeFebruary 17, 2021Luca Minati et al.temperature. Subsequently, proteins were digested at room temperature with 0.5 μg Lys-C (Promega, #VA1170) for 4 hr, after which the solution was diluted four times in 50 mM ammonium bicarbonate. Then, 1 μg

(18) Coordinate Regulation of Ribosome and tRNA Biogenesis Controls Hypoxic Injury and TranslationCurrent BiologyJanuary 1, 2021Omar A. Itani et al.Peptides, and Recombinant Proteins","Chemicals, Peptides, and Recombinant Proteins" Lys-C,Promega,Cat#: VA1170 C18 Sep-Pak solid phase extraction cartridges,Waters,Cat#: WAT036945 Endoproteinase LysC,New England Biolabs

(19) MaTAR25 lncRNA regulates the Tensin1 gene to impact breast cancer progressionNature communicationsDecember 22, 2020Chang KC, Diermeier SD, Yu AT, Brine LD, Russo S, Bhatia S, Alsudani H, Kostroff K, Bhuiya T, Brogi E, et al.10 mM iodoacetamide for 30 min at 55 °C, then digested overnight at 37 °C with 1 μg Lys-C (Promega, VA1170) and dried in vacuo. Peptides were then reconstituted in 50 μl of 0.5 M TEAB/70% ethanol and labeled

(20) Noise Exposures Causing Hearing Loss Generate Proteotoxic Stress and Activate the Proteostasis NetworkCell reportsNovember 24, 2020Jongkamonwiwat N, Ramirez MA, Edassery S, Wong ACY, Yu J, Abbott T, Pak K, Ryan AF, Savas JN.87793 ProteasMax,Promega,Cat# V2071 Sequencing grade modified Trypsin,Promega,Cat# V5111 Lys-C,Promega,Cat# VA1170 Pierce Trypsin Protease,Pierce,Cat# 90058 Casein Blocking Buffer,Sigma,Cat# B6429 "SpikeTides TQL

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